Multicenter Clinical Evaluation of the Automated Aries Bordetella Assay.

ABSTRACT: Molecular methods offer superior sensitivity and specificity and reduce testing turnaround time from days to hours for detection of Bordetella pertussis and Bordetella parapertussis In this study, we evaluated the performance of the automated PCR-based Aries Bordetella Assay, which detects both B. pertussis and B. parapertussis directly from nasopharyngeal swab specimens. The limits of detection (LoDs) were 1,800 CFU·ml^-1 for B. pertussis and 213 CFU·ml^-1 for B. parapertussis The assay detected 16/18 unique B. pertussis/B. parapertussis strains. Of 71 potentially cross-reacting organisms, 5 generated false positives in 1/6 replicates; none of 6 additional Bordetella spp. were erroneously detected. S

SUBMITTER: Relich RF 

PROVIDER: S-EPMC6355524 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature