Increased urinary exosomal microRNAs in children with idiopathic nephrotic syndrome.

ABSTRACT:

Background

Urinary exosomal miRNAs are gaining increasing attention for their potential as ideal non-invasive biomarkers for kidney diseases; however, little is known about their diagnostic ability for paediatric nephrotic syndrome (NS). This study explored the clinical value of urinary exosomal miRNAs for paediatric idiopathic NS.

Methods

Urine samples were collected from 129 NS children and 126 age-/sex-matched healthy controls. The miRNA profile of urinary exosomes was analysed by high-throughput Illumina sequencing via synthesis (SBS) technology followed by verification with a quantitative reverse-transcription polymerase chain reaction (RT-qPCR) assay arranged in two independent cohorts. Additionally, paired urine samples from 65 of these patients were collected before

SUBMITTER: Chen T 

PROVIDER: S-EPMC6355644 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature