Molecular, Biological and Structural Features of V_L CDR-1 Rb44 Peptide, Which Targets the Microtubule Network in Melanoma Cells.

ABSTRACT: Microtubules are important drug targets in tumor cells, owing to their role in supporting and determining the cell shape, organelle movement and cell division. The complementarity-determining regions (CDRs) of immunoglobulins have been reported to be a source of anti-tumor peptide sequences, independently of the original antibody specificity for a given antigen. We found that, the anti-Lewis B mAb light-chain CDR1 synthetic peptide Rb44, interacted with microtubules and induced depolymerization, with subsequent degradation of actin filaments, leading to depolarization of mitochondrial membrane-potential, increase of ROS, cell cycle arrest at G2/M, cleavage of caspase-9, caspase-3 and PARP, upregulation of Bax and downregulation of Bcl-2, altogether resulting in intrinsic apoptosis of melan

SUBMITTER: Girola N 

PROVIDER: S-EPMC6355703 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature