Protosappanin B promotes apoptosis and causes G₁ cell cycle arrest in human bladder cancer cells.

ABSTRACT: The aim of the study was to investigate the effects of protosappanin B on the proliferation and apoptosis of bladder cancer cells. The effects of protosappanin B (12.5, 25, 50, 100, or 200 μg/mL, 48 h) on proliferation of SV-HUC-1, T24 and 5637 cells was assessed using the MTT assay. The effects of protosappanin B (100, 150, 200, 250, or 300 μg/mL, 48 h) on cell apoptosis and cell cycle were analyzed using flow cytometry. T24 and 5637 cells treated with 200 µg/mL protosappanin B showed morphological changes (shrinkage, rounding, membrane abnormalities, and reduced adhesion), but protosappanin B had no proliferation arrest effect on SV-HUC-1 cells. Protosappanin B caused concentration-dependent inhibition of cell growth, with IC₅₀ of 82.78 µg/mL in T24 cells and 113.79 µg/mL in 5

SUBMITTER: Yang X 

PROVIDER: S-EPMC6355918 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature