Integrative single-cell analysis of transcriptome, DNA methylome and chromatin accessibility in mouse oocytes.

ABSTRACT: Oocyte growth is a key step in forming mature eggs that are ready to be fertilized. The states and modifications of chromatin represent critical sources of information for this process. However, the dynamics and interrelations of these chromatin characteristics remain elusive. In this study, we developed an improved scCOOL-seq technique (iscCOOL-seq), which is a multi-omics, single-cell and single-base resolution method with high mapping rates, and explored the chromatin accessibility landscape and its relationship to DNA methylation in growing mouse oocytes. The most dramatic change in chromatin accessibility occurs during oocyte growth initiation, accompanied with prominent transcriptome alterations and an elevated variation in DNA methylation levels among individual oocytes. Unlike CpG

SUBMITTER: Gu C 

PROVIDER: S-EPMC6355938 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature