FlashPack: Fast and Simple Preparation of Ultrahigh-performance Capillary Columns for LC-MS.

ABSTRACT: Capillary ultrahigh-pressure liquid chromatography (cUHPLC) is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We developed a simple and fast method called FlashPack for custom packing of capillary columns of 50-100 cm length with sub- 2 μm sorbent particles. FlashPack uses high sorbent concentrations of 500-1,000 mg/ml for packing at relatively low pressure of 100 bar. Column blocking by sorbent aggregation is avoided during the packing by gentle mechanical tapping of the capillary proximal end by a slowly rotating magnet bar. Utilizing a standard 100-bar pressure bomb, Flashpack allows for production of 15-25 cm cUHPLC columns within a few minutes and of 50 cm cUHPLC columns in less than an hour. Columns exhibit excellent reproducibi

SUBMITTER: Kovalchuk SI 

PROVIDER: S-EPMC6356079 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature