Microfluidic Device for Screening for Target Cell-Specific Binding Molecules by Using Adherent Cells.

ABSTRACT: This paper proposes a microfluidic device for screening molecules such as aptamers, antibodies, proteins, etc. for target cell-specific binding molecules. The discovery of cancer cell-specific binding molecules was the goal of this study. Its functions include filtering non-target cell-binding molecules, trapping molecules on the surface of target cells, washing away unbound molecules, and collecting target cell-specific binding molecules from target cells. These functions were effectively implemented by using our previously developed micro pillar arrays for cell homogeneous dispersion and pneumatic microvalves for tall microchannels. The device was also equipped with serially connected filter chambers in which non-target cells were cultured to reduce the molecules binding to non-target ce

SUBMITTER: Kaminaga M 

PROVIDER: S-EPMC6356270 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature