Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification.

ABSTRACT: Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics. Therefore, a rapid and efficient one-step affinity purification procedure plays important roles in screening native chitosanases. In this study, we report the design and synthesis of affinity resin for efficient purification of native chitosanases without any tags, using chitodisaccharides (CHDS) as an affinity ligand, to couple with Sepharose 6B via a spacer, cyanuric chloride. Based on the CHDS-modified affinity resin, a one-step affinity purification method was developed and optimized, and then applied to purify three typical glycoside hydrolase (GH) fam

SUBMITTER: Li S 

PROVIDER: S-EPMC6356299 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature