Highly Resolved Phylogenetic Relationships within Order Acipenseriformes According to Novel Nuclear Markers.

ABSTRACT: Order Acipenseriformes contains 27 extant species distributed across the northern hemisphere, including so-called "living fossil" species of garfish and sturgeons. Previous studies have focused on their mitochondrial genetics and have rarely used nuclear genetic data, leaving questions as to their phylogenetic relationships. This study aimed to utilize a bioinformatics approach to screen for candidate single-copy nuclear genes, using transcriptomic data from sturgeon species and genomic data from the spotted gar, Lepisosteus oculatus. We utilized nested polymerase chain reaction (PCR) and degenerate primers to identify nuclear protein-coding (NPC) gene markers to determine phylogenetic relationships among the Acipenseriformes. We identified 193 nuclear single-copy genes, selected from 1850

SUBMITTER: Luo D 

PROVIDER: S-EPMC6356338 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature