Integrating 16S rRNA Sequencing and LC⁻MS-Based Metabolomics to Evaluate the Effects of Live Yeast on Rumen Function in Beef Cattle.

ABSTRACT: We evaluated the effects of live yeast on ruminal bacterial diversity and metabolome of beef steer. Eight rumen-cannulated Holstein steers were assigned randomly to one of two treatment sequences in a study with two 25-d experimental periods and a crossover design. The steers were housed in individual pens. The dietary treatments were control (CON) or yeast (YEA; CON plus 15 g/d of live yeast product). Bacterial diversity was examined by sequencing the V3-V4 region of 16S rRNA gene. The metabolome analysis was performed using a liquid chromatograph and a mass spectrometry system (LC⁻MS). Live yeast supplementation increased the relative abundance of eight cellulolytic bacterial genera as well as Anaerovorax and Lachnospiraceae. Proteiniclasticum, Salmonella, and

SUBMITTER: Ogunade I 

PROVIDER: S-EPMC6356510 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature