A Sequence-Based Novel Approach for Quality Evaluation of Third-Generation Sequencing Reads.

ABSTRACT: The advent of third-generation sequencing (TGS) technologies, such as the Pacific Biosciences (PacBio) and Oxford Nanopore machines, provides new possibilities for contig assembly, scaffolding, and high-performance computing in bioinformatics due to its long reads. However, the high error rate and poor quality of TGS reads provide new challenges for accurate genome assembly and long-read alignment. Efficient processing methods are in need to prioritize high-quality reads for improving the results of error correction and assembly. In this study, we proposed a novel Read Quality Evaluation and Selection Tool (REQUEST) for evaluating the quality of third-generation long reads. REQUEST generates training data of high-quality and low-quality reads which are characterized by their nucleotide com

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6356754 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature