Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures.

ABSTRACT: Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system's technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific

SUBMITTER: Reyes M 

PROVIDER: S-EPMC6357748 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature