Establishing a detection method for CCNY: a potentially significant clinical investigative marker in NSCLC patients.

ABSTRACT:

Background

CCNY, a novel cyclin family member, plays an increasingly important role in the progression of tumor invasion and metastasis, including lung cancer. However, the clinical significance of CCNY in non-small-cell lung cancer (NSCLC) patients is unknown.

Patients and methods

We prepared CCNY monoclonal antibodies, validated specific peptides by a peptide array, and established a double-antibody sandwich ELISA detection method. Then, we measured CCNY levels in 100 NSCLC patients and 50 healthy controls. A blinded validation was subsequently performed in 399 NSCLC patients and 150 healthy controls.

Results

We successfully prepared two specific mouse anti-human CCNY monoclonal antibodies and established a reliable and stable detection method. In the training set,

SUBMITTER: Ma L 

PROVIDER: S-EPMC6357874 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature