Reversing platinum resistance in ovarian cancer multicellular spheroids by targeting Bcl-2.

ABSTRACT:

Purpose

Peritoneal metastasis is the most common pathway for the spread of ovarian cancer. Ovarian cancer cells in ascites prefer to aggregate into the more chemoresistant multicellular spheroids (MCSs), leading to treatment failure and disease recurrence. We previously established a suspension MCS model of ovarian cancer cells in vitro and found that the MCS cells acquired drug resistance to cisplatin. In the present study, we aimed to uncover the underlying mechanism of the platinum resistance of MCS and the potential targets to reverse the drug resistance.

Materials and methods

MCS models were established for the phenotypic studies, including proliferation, invasion, migration, drug resistance, apoptosis assays, and signaling pathway analysis. The key molecule, Bcl-2, was

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6357888 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature