Relative Frequencies of Alloantigen-Specific Helper CD4 T Cells and B Cells Determine Mode of Antibody-Mediated Allograft Rejection.

ABSTRACT: Humoral alloimmunity is now recognized as a major determinant of transplant outcome. MHC glycoprotein is considered a typical T-dependent antigen, but the nature of the T cell alloresponse that underpins alloantibody generation remains poorly understood. Here, we examine how the relative frequencies of alloantigen-specific B cells and helper CD4 T cells influence the humoral alloimmune response and how this relates to antibody-mediated rejection (AMR). An MHC-mismatched murine model of cardiac AMR was developed, in which T cell help for alloantibody responses in T cell deficient (Tcrbd^-/-) C57BL/6 recipients against donor H-2K^d MHC class I alloantigen was provided by adoptively transferred "TCR75" CD4 T cells that recognize processed H-2K^d allopeptid

SUBMITTER: Alsughayyir J 

PROVIDER: S-EPMC6357941 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature