Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria.

ABSTRACT:

Background

The prompt diagnosis of plasmodial species for effective treatment prevents worsening of individual health and avoids transmission maintenance or even malaria reintroduction in areas where Plasmodium does not exist. Polymerase chain reaction (PCR) allows for the detection of parasites below the threshold of microscopic examination.

Objective

Our aim was to develop a real-time PCR test to reduce diagnostic errors and increase efficacy.

Methods

The lower limit of quantification and the linearity/analytical sensitivity to measure sensitivity or limit of detection (LoD) were determined. Intra-assay variations (repeatability) and alterations between assays, operators, and instruments (reproducibility) were also assessed to set precision.

Findings

The line

SUBMITTER: Almeida-de-Oliveira NK 

PROVIDER: S-EPMC6358008 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature