Transition from a meiotic to a somatic-like DNA damage response during the pachytene stage in mouse meiosis.

ABSTRACT: Homologous recombination (HR) is the principal mechanism of DNA repair acting during meiosis and is fundamental for the segregation of chromosomes and the increase of genetic diversity. Nevertheless, non-homologous end joining (NHEJ) mechanisms can also act during meiosis, mainly in response to exogenously-induced DNA damage in late stages of first meiotic prophase. In order to better understand the relationship between these two repair pathways, we studied the response to DNA damage during male mouse meiosis after gamma radiation. We clearly discerned two types of responses immediately after treatment. From leptotene to early pachytene, exogenous damage triggered the massive presence of γH2AX throughout the nucleus, which was associated with DNA repair mediated by HR components (DMC1 and

SUBMITTER: Enguita-Marruedo A 

PROVIDER: S-EPMC6358097 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature