Automated Morphological Feature Assessment for Zebrafish Embryo Developmental Toxicity Screens.

ABSTRACT: Detection of developmental phenotypes in zebrafish embryos typically involves a visual assessment and scoring of morphological features by an individual researcher. Subjective scoring could impact results and be of particular concern when phenotypic effect patterns are also used as a diagnostic tool to classify compounds. Here we introduce a quantitative morphometric approach based on image analysis of zebrafish embryos. A software called FishInspector was developed to detect morphological features from images collected using an automated system to position zebrafish embryos. The analysis was verified and compared with visual assessments of 3 participating laboratories using 3 known developmental toxicants (methotrexate, dexamethasone, and topiramate) and 2 negative compounds (loratadine a

SUBMITTER: Teixido E 

PROVIDER: S-EPMC6358258 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature