Protein and lipid fingerprinting of native-like membrane complexes by combining TLC and protein electrophoresis.

ABSTRACT: TLC has traditionally been used to analyze lipids isolated from membrane complexes. Here, we describe a method based on the combination of TLC and SDS-PAGE to qualitatively analyze the protein/lipid profile of membrane complexes such as those of lung surfactant. For this purpose, native lung surfactant was applied onto a silica TLC plate in the form of an aqueous suspension, preserving not only hydrophilic proteins associated with lipids but also native protein-lipid interactions. Using native membrane complexes in TLC allows the differential migration of lipids and their separation from the protein components. As a result, (partly) delipidated protein-enriched bands can be visualized and analyzed by SDS-PAGE to identify proteins originally associated with lipids. Interestingly, the hydrop

SUBMITTER: Lopez-Rodriguez E 

PROVIDER: S-EPMC6358299 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature