PET imaging of EGFR expression using an ¹⁸F-labeled RNA aptamer.

ABSTRACT:

Objective

Epidermal growth factor receptor (EGFR) is a theranostic biomarker for a variety of cancer types. The aim of the present study was to develop an ¹⁸F radiolabeled EGFR targeting RNA aptamer, and to investigate its ability to visualize and quantify EGFR in xenograft models.

Methods

Biolayer interferometry binding assay was used to detect the binding affinity of the alkyne-modified EGFR aptamer MinE07 (denoted as ME07) with recombinant human wild-type EGFR protein and the mutant EGFRvIII protein. Cy5-conjugated ME07 was used for flow cytometry and immunofluorescence staining, and an Alexa Fluor 488-labeled EGFR antibody (ab193244) was used as a control. ¹⁸F-Fluorobenzoyl (FB) azide was employed as a synthon to produce ¹⁸F-FB-ME07 via c

SUBMITTER: Cheng S 

PROVIDER: S-EPMC6358511 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature