Preadipocytes of obese humans display gender-specific bioenergetic responses to glucose and insulin.

ABSTRACT:

Background/objectives

Although the prevalence of obesity and its associated metabolic disorders is increasing in both sexes, the clinical phenotype differs between men and women, highlighting the need for individual treatment options. Mitochondrial dysfunction in various tissues, including white adipose tissue (WAT), has been accepted as a key factor for obesity-associated comorbidities such as diabetes. Given higher expression of mitochondria-related genes in the WAT of women, we hypothesized that gender differences in the bioenergetic profile of white (pre-) adipocytes from obese (age- and BMI-matched) donors must exist.

Subjects/methods

Using Seahorse technology, we measured oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of (pre-)adipocytes fr

SUBMITTER: Keuper M 

PROVIDER: S-EPMC6358537 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature