Regulation of glucose uptake and inflammation markers by FOXO1 and FOXO3 in skeletal muscle.

ABSTRACT:

Objective

Forkhead box class O (FOXO) transcription factors regulate whole body energy metabolism, skeletal muscle mass, and substrate switching. FOXO1 and FOXO3 are highly abundant transcription factors, but their precise role in skeletal muscle metabolism has not been fully elucidated.

Methods

To elucidate the role of FOXO in skeletal muscle, dominant negative (dn) constructs for FOXO1 (FOXO1dn) or FOXO3 (FOXO3dn) were transfected by electroporation into mouse tibialis anterior muscle and glucose uptake, signal transduction, and gene expression profiles were assessed after an oral glucose tolerance test. Results were compared against contralateral control transfected muscle.

Results

FOXO1dn and FOXO3dn attenuated glucose uptake (35%, p < 0.01 and 20%, p < 0.05), GLU

SUBMITTER: Lundell LS 

PROVIDER: S-EPMC6358548 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature