On-demand serum-free media formulations for human hematopoietic cell expansion using a high dimensional search algorithm.

ABSTRACT: Substitution of serum and other clinically incompatible reagents is requisite for controlling product quality in a therapeutic cell manufacturing process. However, substitution with chemically defined compounds creates a complex, large-scale optimization problem due to the large number of possible factors and dose levels, making conventional process optimization methods ineffective. We present a framework for high-dimensional optimization of serum-free formulations for the expansion of human hematopoietic cells. Our model-free approach utilizes evolutionary computing principles to drive an experiment-based feedback control platform. We validate this method by optimizing serum-free formulations for first, TF-1 cells and second, primary T-cells. For each cell type, we successfully identify a

SUBMITTER: Kim MM 

PROVIDER: S-EPMC6358607 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature