Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry.

ABSTRACT: Total fatty acid analysis is a routine method in many areas, including lipotyping of individuals in personalized medicine, analysis of foodstuffs, and optimization of oil production in biotechnology. This analysis is commonly done by converting fatty acyl (FA) chains of intact lipids into FA methyl esters (FAMEs) and monitoring these by gas-chromatography (GC)-based methods, typically requiring at least 15 min of analysis per sample. Here, we describe a novel method that supports fast, precise and accurate absolute quantification of total FA levels in human plasma and serum samples. The method uses acid-catalyzed transesterification with ¹⁸O-enriched H₂O (i.e., H₂¹⁸O) to convert FA chains into ¹⁸O-labeled free fatty acids. The resulting "mass-tagged" FA ana

SUBMITTER: Gallego SF 

PROVIDER: S-EPMC6359376 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature