TRIM4 is associated with neural tube defects based on genome-wide DNA methylation analysis.

ABSTRACT:

Background

Neural tube defects (NTDs) are complex abnormalities associated with gene-environment interactions. The underlying cause has not been determined.

Methods

Spinal cord tissues from cases with NTDs and healthy controls were collected. Methylation patterns between cases and normal individuals were compared using 450K Infinium Methylation BeadChip Illumina. DNA methylation analysis by pyrosequencing (PyroMark Q96) and mRNA and protein expression were analyzed using real-time quantitative PCR and Western blotting, respectively. Next-generation and Sanger sequencing were used to determine genetic variants in the target genes.

Results

Spinal cord tissues from cases with NTDs had more hypomethylated than hypermethylated genes. Further evaluation showed that the exon

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6359777 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature