Influence of total polar compounds on lipid metabolism, oxidative stress and cytotoxicity in HepG2 cells.

ABSTRACT:

Background

Recently, the harmful effects of frying oil on health have been gradually realized. However, as main components of frying oils, biochemical effects of total polar compounds (TPC) on a cellular level were underestimated.

Methods

The effects of total polar compounds (TPC) in the frying oil on the lipid metabolism, oxidative stress and cytotoxicity of HepG2 cells were investigated through a series of biochemical methods, such as oil red staining, real-time polymerase chain reaction (RT-PCR), cell apoptosis and cell arrest.

Results

Herein, we found that the survival rate of HepG2 cells treated with TPC decreased in a time and dose dependent manner, and thereby presented significant lipid deposition over the concentration of 0.5 mg/mL. TPC were also found to sup

SUBMITTER: Ju J 

PROVIDER: S-EPMC6359786 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature