Proteomic analysis of plasma exosomes to differentiate malignant from benign pulmonary nodules.

ABSTRACT:

Background

It is difficult to distinguish benign pulmonary nodules (PNs) from malignant PNs by conventional examination. Therefore, novel biomarkers that can identify the nature of PNs are needed. Exosomes have recently been identified as an attractive alternative approach since tumor-specific molecules can be found in exosomes isolated from biological fluids.

Methods

Plasma exosomes were extracted via the exoEasy reagent method. The major proteins from plasma exosomes in patients with PNs were identified via labelfree analysis and screened for differentially expressed proteins. A GO classification analysis and KEGG pathway analysis were performed on plasma exosomal protein from patients with benign and malignant PNs.

Results

Western blot confirmed that protein expres

SUBMITTER: Kuang M 

PROVIDER: S-EPMC6359787 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature