Protein Biomarkers and Neuroproteomics Characterization of Microvesicles/Exosomes from Human Cerebrospinal Fluid Following Traumatic Brain Injury.

ABSTRACT: Recently, there have been emerging interests in the area of microvesicles and exosome (MV/E) released from brain cells in relation to neurodegenerative diseases. However, only limited studies focused on MV/E released post-traumatic brain injury (TBI) as they highlight on the mechanistic roles of released proteins. This study sought to examine if CSF samples from severe TBI patients contain MV/E with unique protein contents. First, nanoparticle tracking analysis determined MV/E from TBI have a mode of 74-98 nm in diameter, while control CSF MV/E have a mode of 99-104 nm. Also, there are more MV/E were isolated from TBI CSF (27.8-33.6 × 10⁸/mL) than from control CSF (13.1-18.5 × 10⁸/mL). Transmission electron microscopy (TEM) visualization also confirmed characteristic

SUBMITTER: Manek R 

PROVIDER: S-EPMC6359938 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature