Validation of reference genes for gene expression studies in post-harvest leaves of tea plant (Camellia sinensis).

ABSTRACT: Tea is one of three major non-alcoholic beverages that are popular all around the world. The economic value of tea product largely depends on the post-harvest physiology of tea leaves. The utilization of quantitative reverse transcription polymerase chain reaction is a widely accepted and precise approach to determine the target gene expression of tea plants, and the reliability of results hinges on the selection of suitable reference genes. A few reliable reference genes have been documented using various treatments and different tissues of tea plants, but none has been done on post-harvest leaves during the tea manufacturing process. The present study selected and analyzed 15 candidate reference genes: Cs18SrRNA, CsGADPH, CsACT, CsEF-1α, CsUbi, CsTUA

SUBMITTER: Zhou ZW 

PROVIDER: S-EPMC6360078 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature