Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers.

ABSTRACT: Epithelial cells and differentiated fiber cells represent distinct compartments in the ocular lens. While previous studies have revealed proteins that are preferentially expressed in epithelial vs. fiber cells, a comprehensive proteomics library comparing the molecular compositions of epithelial vs. fiber cells is essential for understanding lens formation, function, disease and regenerative potential, and for efficient differentiation of pluripotent stem cells for modeling of lens development and pathology in vitro. To compare protein compositions between the lens epithelium and fibers, we employed tandem mass spectrometry (2D-LC/MS) analysis of microdissected mouse P0.5 lenses. Functional classifications of the top 525 identified proteins into gene ontology categories by molecular proces

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC6360118 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature