Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds.

ABSTRACT:

Introduction

The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.

Methods

Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.

Results

In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thicknes

SUBMITTER: Kaita Y 

PROVIDER: S-EPMC6360408 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature