Inhibitory effects of class I antiarrhythmic agents on Na⁺ and Ca²⁺ currents of human iPS cell-derived cardiomyocytes.

ABSTRACT:

Introduction

Human induced pluripotent stem cells (hiPSCs) harboring cardiac myosin heavy chain 6 promoter can differentiate into functional cardiomyocytes called "iCell cardiomyocytes" under blasticidin treatment condition. While iCell cardiomyocytes are expected to be used for predicting cardiotoxicity of drugs, their responses to antiarrhythmic agents remain to be elucidated. We first examined electrophysiological properties of iCell cardiomyocytes and mRNA levels of ion channels and Ca handling proteins, and then evaluated effects of class I antiarrhythmic agents on their Na⁺ and Ca²⁺ currents.

Methods

iCell cardiomyocytes were cultured for 8-14 days (38-44 days after inducing their differentiation), according to the manufacturer's protocol. We dete

SUBMITTER: Yonemizu S 

PROVIDER: S-EPMC6360514 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature