CeL-ID: cell line identification using RNA-seq data.

ABSTRACT:

Background

Cell lines form the cornerstone of cell-based experimentation studies into understanding the underlying mechanisms of normal and disease biology including cancer. However, it is commonly acknowledged that contamination of cell lines is a prevalent problem affecting biomedical science and available methods for cell line authentication suffer from limited access as well as being too daunting and time-consuming for many researchers. Therefore, a new and cost effective approach for authentication and quality control of cell lines is needed.

Results

We have developed a new RNA-seq based approach named CeL-ID for cell line authentication. CeL-ID uses RNA-seq data to identify variants and compare with variant profiles of other cell lines. RNA-seq data for 934 CCLE cell l

SUBMITTER: Mohammad TA 

PROVIDER: S-EPMC6360649 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature