NanoMod: a computational tool to detect DNA modifications using Nanopore long-read sequencing data.

ABSTRACT:

Background

Recent advances in single-molecule sequencing techniques, such as Nanopore sequencing, improved read length, increased sequencing throughput, and enabled direct detection of DNA modifications through the analysis of raw signals. These DNA modifications include naturally occurring modifications such as DNA methylations, as well as modifications that are introduced by DNA damage or through synthetic modifications to one of the four standard nucleotides.

Methods

To improve the performance of detecting DNA modifications, especially synthetically introduced modifications, we developed a novel computational tool called NanoMod. NanoMod takes raw signal data on a pair of DNA samples with and without modified bases, extracts signal intensities, performs base error correct

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6360650 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature