Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells.

ABSTRACT:

Background

Differentiation of induced pluripotent stem cells (iPSCs) toward hematopoietic progenitor cells (HPCs) raises high hopes for disease modeling, drug screening, and cellular therapy. Various differentiation protocols have been established to generate iPSC-derived HPCs (iHPCs) that resemble their primary counterparts in morphology and immunophenotype, whereas a systematic epigenetic comparison was yet elusive.

Results

In this study, we compared genome-wide DNA methylation (DNAm) patterns of iHPCs with various different hematopoietic subsets. After 20 days of in vitro differentiation, cells revealed typical hematopoietic morphology, CD45 expression, and colony-forming unit (CFU) potential. DNAm changes were particularly observed in genes that are associated with hemat

SUBMITTER: Cypris O 

PROVIDER: S-EPMC6360658 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature