Identification of the targets of hematoporphyrin derivative in lung adenocarcinoma using integrated network analysis.

ABSTRACT:

Background

Hematoporphyrin derivative (HPD) has a sensibilization effect in lung adenocarcinoma. This study was conducted to identify the target genes of HPD in lung adenocarcinoma.

Methods

RNA sequencing was performed using the lung adenocarcinoma cell line A549 after no treatment or treatment with X-ray or X-ray + HPD. The differentially expressed genes (DEGs) were screened using Mfuzz package by noise-robust soft clustering analysis. Enrichment analysis was carried out using "BioCloud" online tool. Protein-protein interaction (PPI) network and module analyses were performed using Cytoscape software. Using WebGestalt tool and integrated transcription factor platform (ITFP), microRNA target and transcription factor (TF) target pairs were separately predicted. An integrated

SUBMITTER: Yin H 

PROVIDER: S-EPMC6360726 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature