A dynamic and integrated epigenetic program at distal regions orchestrates transcriptional responses to VEGFA.

ABSTRACT: Cell behaviors are dictated by epigenetic and transcriptional programs. Little is known about how extracellular stimuli modulate these programs to reshape gene expression and control cell behavioral responses. Here, we interrogated the epigenetic and transcriptional response of endothelial cells to VEGFA treatment and found rapid chromatin changes that mediate broad transcriptomic alterations. VEGFA-responsive genes were associated with active promoters, but changes in promoter histone marks were not tightly linked to gene expression changes. VEGFA altered transcription factor occupancy and the distal epigenetic landscape, which profoundly contributed to VEGFA-dependent changes in gene expression. Integration of gene expression, dynamic enhancer, and transcription factor occupancy changes

SUBMITTER: Wang S 

PROVIDER: S-EPMC6360815 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature