MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network.

ABSTRACT: Gene expression can be regulated at multiple levels, but it is not known if and how there is broad coordination between regulation at the transcriptional and post-transcriptional levels. Transcription factors and chromatin regulate gene expression transcriptionally, whereas microRNAs (miRNAs) are small regulatory RNAs that function post-transcriptionally. Systematically identifying the post-transcriptional targets of miRNAs and the mechanism of transcriptional regulation of the same targets can shed light on regulatory networks connecting transcriptional and post-transcriptional control. We used individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) for the RNA-induced silencing complex (RISC) component AGO2 and global miRNA depletion to identify genes directly ta

SUBMITTER: Shivram H 

PROVIDER: S-EPMC6360819 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature