Dataset Information

A gelatin/collagen/polycaprolactone scaffold for skin regeneration.

ABSTRACT:

Background

A tissue-engineered skin substitute, based on gelatin ("G"), collagen ("C"), and poly(ε-caprolactone) (PCL; "P"), was developed.

Method

G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used.

Results

Differential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 °C. Scanning electron microscopy showed that all biocomposites had similar fibrous structures. Good cytocompatibility was present in all G/C/P biocomposites when incubated with primary human epidermal keratinocytes (PHEK), human dermal fibroblasts (PHDF) and human adipose-derived stem cells (ASCs) in vitro. All G/C/P biocomposites exhibited similar cell growth and mechanical characteristics in comparison with C/P biocomposites. G/C/P biocomposites with a lower collagen content showed better cell proliferation than those with a higher collagen content in vitro. Due to reasonable mechanical strength and biocompatibility in vitro, G/C/P with a lower content of collagen and a higher content of PCL (GC_LP_H) was selected for animal wound healing studies. According to our data, a significant promotion in wound healing and skin regeneration could be observed in GC_LP_H seeded with adipose-derived stem cells by Gomori's trichrome staining.

Conclusion

This study may provide an effective and low-cost wound dressings to assist skin regeneration for clinical use.

SUBMITTER: Wei LG

PROVIDER: S-EPMC6361006 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A gelatin/collagen/polycaprolactone scaffold for skin regeneration.

Wei Lin-Gwei LG Chang Hsin-I HI Wang Yiwei Y Hsu Shan-Hui SH Dai Lien-Guo LG Fu Keng-Yen KY Dai Niann-Tzyy NT

PeerJ 20190201

<h4>Background</h4>A tissue-engineered skin substitute, based on gelatin ("G"), collagen ("C"), and poly(ε-caprolactone) (PCL; "P"), was developed.<h4>Method</h4>G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used.<h4>Results</h4>Differential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around ...[more]

PMID: 30723629

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Dataset Information

A gelatin/collagen/polycaprolactone scaffold for skin regeneration.

Background

Method

Results

Conclusion

Publications

A gelatin/collagen/polycaprolactone scaffold for skin regeneration.

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