A chemical-genetics approach to study the role of atypical Protein Kinase C in <i>Drosophila</i>.

ABSTRACT: Studying the function of proteins using genetics in cycling cells is complicated by the fact that there is often a delay between gene inactivation and the time point of phenotypic analysis. This is particularly true when studying kinases that have pleiotropic functions and multiple substrates. Drosophila neuroblasts (NBs) are rapidly dividing stem cells and an important model system for the study of cell polarity. Mutations in multiple kinases cause NB polarity defects, but their precise functions at particular time points in the cell cycle are unknown. Here, we use chemical genetics and report the generation of an analogue-sensitive allele of Drosophila atypical Protein Kinase C (aPKC). We demonstrate that the resulting mutant aPKC kinase can be specifically inhibited in

SUBMITTER: Hannaford M 

PROVIDER: S-EPMC6361133 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature