Modelling pancreatic β-cell inflammation in zebrafish identifies the natural product wedelolactone for human islet protection.

ABSTRACT: Islet inflammation and cytokine production are implicated in pancreatic β-cell dysfunction and diabetes pathogenesis. However, we lack therapeutics to protect the insulin-producing β-cells from inflammatory damage. Closing this clinical gap requires the establishment of new disease models of islet inflammation to facilitate screening efforts aimed at identifying new protective agents. Here, we have developed a genetic model of Interleukin-1β (Il-1β)-driven islet inflammation in zebrafish, a vertebrate that allows for non-invasive imaging of β-cells and in vivo drug discovery. Live imaging of immune cells and β-cells in our model revealed dynamic migration, increased visitation and prolonged macrophage retention in the islet, together with robust activation of NF-κB signalling in β-c

SUBMITTER: Delgadillo-Silva LF 

PROVIDER: S-EPMC6361155 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature