Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF.

ABSTRACT: The diffusible signal factors (DSFs) are a family of quorum-sensing autoinducers (AIs) produced and detected by numerous gram-negative bacteria. The DSF family AIs are fatty acids, differing in their acyl chain length, branching, and substitution but having in common a cis-2 double bond that is required for their activity. In both human and plant pathogens, DSFs regulate diverse phenotypes, including virulence factor expression, antibiotic resistance, and biofilm dispersal. Despite their widespread relevance to both human health and agriculture, the molecular basis of DSF recognition by their cellular receptors remained a mystery. Here, we report the first structure-function studies of the DSF receptor regulation of pathogenicity factor R (RpfR). We present the X-ray crystal structure of t

SUBMITTER: Waldron EJ 

PROVIDER: S-EPMC6361424 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature