Comprehensive profiling of translation initiation in influenza virus infected cells.

ABSTRACT: Translation can initiate at alternate, non-canonical start codons in response to stressful stimuli in mammalian cells. Recent studies suggest that viral infection and anti-viral responses alter sites of translation initiation, and in some cases, lead to production of novel immune epitopes. Here we systematically investigate the extent and impact of alternate translation initiation in cells infected with influenza virus. We perform evolutionary analyses that suggest selection against non-canonical initiation at CUG codons in influenza virus lineages that have adapted to mammalian hosts. We then use ribosome profiling with the initiation inhibitor lactimidomycin to experimentally delineate translation initiation sites in a human lung epithelial cell line infected with influenza virus. We ide

SUBMITTER: Machkovech HM 

PROVIDER: S-EPMC6361465 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature