Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR.
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ABSTRACT:
SUBMITTER: Goto Y
PROVIDER: S-EPMC6361656 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
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Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR.
Goto Yusuke Y Yaegashi Gakuji G Kumagai Yoshihiro Y Ogasawara Fusae F Goto Makiko M Mase Masaji M
The Journal of veterinary medical science 20181121 1
A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5'-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV v ...[more]
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