High doses of synthetic antioxidants induce premature senescence in cultivated mesenchymal stem cells.

ABSTRACT: Stress-induced premature senescence program is known to be activated in cells by various genotoxic stressors, and oxidative stress is considered to be the main of those. To this end, many studies discover antioxidants as protective anti-aging agents. In the current study, we examined the effects of different antioxidants (Tempol, resveratrol, NAC, DPI) on the mesenchymal stem cells maintained in normal physiological conditions. We used high, but non-cytotoxic antioxidant doses which are widely used in laboratory practice to protect cells from oxidative damage. We show that these substances induce reversible block of cell proliferation and do not cause any genotoxic effects when applied to the quiescent cells. However, the same doses of the same substances, when applied to the proliferating

SUBMITTER: Kornienko JS 

PROVIDER: S-EPMC6361906 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature