A continuous fluorescence assay for simple quantification of bile salt hydrolase activity in the gut microbiome.

ABSTRACT: The microbiota of the mammalian gut plays a dynamic role in controlling host physiology. The effect of gut microbiota activity on host health is particularly evident in the case of bile homeostasis. Bile is produced by the host and is modified by the gut microbiota, which impacts the net hydrophobicity of the total bile acid pool, and also modulates host signaling pathways. A key mechanism by which the microbiota modify bile is through deconjugation of bile salts through bile salt hydrolase (BSH) enzymatic activity, which is postulated to be a prerequisite for all further microbial metabolism. BSH activity in the gut is largely considered to be beneficial for the host, and genes encoding BSHs are found in the genomes of many taxa found in over-the-counter probiotics. Despite the therapeuti

SUBMITTER: Brandvold KR 

PROVIDER: S-EPMC6361941 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature