Inferring Gene Regulatory Networks from a Population of Yeast Segregants.

ABSTRACT: Constructing gene regulatory networks is crucial to unraveling the genetic architecture of complex traits and to understanding the mechanisms of diseases. On the basis of gene expression and single nucleotide polymorphism data in the yeast, Saccharomyces cerevisiae, we constructed gene regulatory networks using a two-stage penalized least squares method. A large system of structural equations via optimal prediction of a set of surrogate variables was established at the first stage, followed by consistent selection of regulatory effects at the second stage. Using this approach, we identified subnetworks that were enriched in gene ontology categories, revealing directional regulatory mechanisms controlling these biological pathways. Our mapping and analysis of expression-based quantitative t

SUBMITTER: Chen C 

PROVIDER: S-EPMC6361976 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature