Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.

ABSTRACT: The kidney possesses a highly organised vasculature that is required for its filtration function. While recent advances in stem cell biology have enabled the in vitro generation of kidney tissues, at least partially, recapitulation of the complicated vascular architecture remains a huge challenge. Herein we develop a method to reconstitute both the kidney and its vascular architecture in vitro, using dissociated and sorted mouse embryonic kidney cells. Upon transplantation, arteriolar networks were re-established that ran through the interstitial space between branching ureteric buds and eventually entered glomeruli. Using this system, we found that donor-derived endothelial cells significantly contributed to the arterioles and glomerular capillaries formed after transplantation. Unexpecte

SUBMITTER: Murakami Y 

PROVIDER: S-EPMC6362047 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature