A step towards valid detection and quantification of lung cancer volume in experimental mice with contrast agent-based X-ray microtomography.

ABSTRACT: Tumor volume is a parameter used to evaluate the performance of new therapies in lung cancer research. Conventional methods that are used to estimate tumor size in mouse models fail to provide fast and reliable volumetric data for tumors grown non-subcutaneously. Here, we evaluated the use of iodine-staining combined with micro-computed tomography (micro-CT) to estimate the tumor volume of ex vivo tumor-burdened lungs. We obtained fast high spatial resolution three-dimensional information of the lungs, and we demonstrated that iodine-staining highlights tumors and unhealthy tissue. We processed iodine-stained lungs for histopathological analysis with routine hematoxylin and eosin (H&E) staining. We compared the traditional tumor burden estimation performed manually with H&E histological sl

SUBMITTER: Bidola P 

PROVIDER: S-EPMC6362109 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature