Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria.

ABSTRACT: Comparative genomic studies of the bacterial MFS-type copper importer CcoA, required for cbb₃-type cytochrome c oxidase (cbb₃-Cox) biogenesis, revealed a widespread CcoA-like transporters (CalT) family, containing the conserved CcoA Cu-binding MxxxM and HxxxM motifs. Surprisingly, this family also included the RfnT-like proteins, earlier suggested to transport riboflavin. However, presence of the Cu-binding motifs in these proteins raised the possibility that they might be Cu transporters. To test this hypothesis, the genomic context of the corresponding genes was examined, and three of such genes from Ochrobactrum anthropi, Rhodopseudomonas palustris and Agrobacterium tumefaciens were expressed in Escherichia coli (ΔribB) and Rhodobacter capsulatus (ΔccoA) mutants. C

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6362234 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature